[PDF] https://doi.org/10.3952/physics.v58i4.3874

Open access article / Atviros prieigos straipsnis
Lith. J. Phys. 58, 295–306 (2018)
 

DECAY- AND EVOLUTION-ASSOCIATED SPECTRA OF TIME-RESOLVED FLUORESCENCE OF LHCII AGGREGATES
 Andrius Gelzinisa,b, Yakov Bravera,b, Jevgenij Chmeliova,b, and Leonas Valkunasa,b
 aInstitute of Chemical Physics, Faculty of Physics, Vilnius University, Saulėtekio 9, 10222 Vilnius, Lithuania
 bDepartment of Molecular Compound Physics, Center for Physical Sciences and Technology, Saulėtekio 3, 10257 Vilnius, Lithuania
 E-mail: leonas.valkunas@ff.vu.lt
Received 22 July 2018; accepted 15 October 2018

Non-photochemical quenching (NPQ) is responsible for the protection of the photosynthetic apparatus of plants from photodamage at high-light conditions. It is commonly agreed that NPQ takes place in the major light-harvesting complexes (LHCII), however, its exact mechanisms are still under debate. Valuable information about its molecular nature can be provided by measuring time-resolved fluorescence (TRF) spectra of LHCII complexes and their aggregates. Previously [Chmeliov et al., Nat. Plants 2, 16045 (2016)], we analysed the corresponding TRF spectra using the multivariate curve resolution method and proposed a three-state model to describe the spectroscopic data. Usually, such data is described in terms of global analysis resulting in decay or evolution-associated spectra. In this work, we apply such analysis to the TRF data of LHCII aggregates and show that, although mathematically feasible, it cannot be directly related to the physical kinetic model. Nevertheless, a careful examination supplemented with additional spectroscopic information still results in the same three-state model proposed before.
Keywords: time-resolved fluorescence, decay associated spectra, ligh-harvesting complex
PACS: 78.47.D-, 78.47.jd, 82.20.Wt

LHCII AGREGATŲ LAIKINĖS SKYROS FLUORESCENCIJOS GESIMO IR EVOLIUCIJOS SPEKTRAI
Andrius Gelžinisa,b, Yakov Bravera,b, Jevgenij Chmeliova,b, Leonas Valkūnasa,b

aVilniaus universiteto Fizikos fakulteto Cheminės fizikos institutas, Vilnius, Lietuva
bFizinių ir technologijos mokslų centro Molekulinių darinių fizikos skyrius, Vilnius, Lietuva
 
Laikinės skyros fluorescencijos spektrų matavimas yra vienas iš populiariausių eksperimentinių metodų, taikomų siekiant išsiaiškinti įvairiose molekulinėse sistemose vykstančių fotoindukuotų vyksmų dinamiką ir ją nulemiančius fizikinius mechanizmus. Neseniai publikuotame darbe [Chmeliov et al., Nat. Plants 2, 16045 (2016)] buvo pristatyti augalų fotosintetinių pigmentų ir baltymų anteninių kompleksų (LHCII) agregatų laikinės skyros fluorescencijos spektrai, išmatuoti esant plačiam temperatūriniam intervalui, o šių duomenų išsami analizė leido atskleisti už nefotocheminį gesinimą atsakingo augalų fotoapsaugos mechanizmo molekulinę prigimtį. Laikinės skyros spektrams interpretuoti plačiai taikomi globaliosios analizės metodai (tokie kaip gesimo ar evoliucijos spektrų skaičiavimas), tačiau priklausomai nuo tiriamos sistemos sudėtingumo jie ne visada sukuria teisingą toje sistemoje vykstančių reiškinių fizikinį vaizdinį. Darbe šie metodai pritaikomi minėtiems LHCII agregatų laikinės skyros fluorescencijos spektrams aprašyti. Parodoma, kad nors pateikia­mi matematiškai teisingi eksperimentinių duomenų aprašymai, dėl sistemos nehomogeniškumo tinkamai interpretuoti galima tik atsižvelgiant į papildomus spekt­roskopinius duo­menis.

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